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KMID : 1035320160430010091
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Korean Society for Dental Materials
2016 Volume.43 No. 1 p.91 ~ p.99
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Agar Overlay Test of Root Canal Sealers Before and After Setting Procedures
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Kim Mi-Joo
Kang Hyo-Jin
Hong Ju-Hee
Kim Kwang-Man
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Abstract
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This study was performed to evaluate the cytotoxicity of root canal sealers before and after setting procedures by an agar overlay test. Six kinds of root canal sealers were used in this experiment: AH Plus, AH 26, ADSEAL, DIA-PROSEAL, Tubli-Seal, and Sealapex. Test materials were prepared as either freshly mixed type of provided components or hardened specimens after setting procedures in each Teflon ring-shaped holder. Agar overlay test was done with L929 mouse fibroblast cells and positive, negative and test materials were set on the solidified agar medium. After 24 hrs, decolorization index and lysis index were evaluated and its cell response was interpretated. Decolorization zones and lysis values by all root canal sealers used in this study showed index 5 when freshly mixed root canal sealers were placed. Decolorization zones by AH Plus and AH 26 were larger than those by other two kinds of resin sealers after setting procedures. Zinc oxide eugenol-based sealer, Tubli-Seal, showed decolorization index 3 and lysis index 5, otherwise, calcium hydroxide based-sealer, Sealapex, did decolorization index 2 and lysis index 4 after hardening procedures. Among test materials, DIA-PROSEAL had the least cytotoxicity after hardening, followed by ADSEAL and Tubli-Seal. In conclusion, all root canal sealers had the severe cytotoxic effects when freshly mixed materials were tested. However, cytotoxicity was reduced after sealer hardening, especially in case of DIA-PROSEAL.
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KEYWORD
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Agar Overlay Test, Cytotoxicity, Root Canal Sealer
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